Objective: Anxiety and Insomnia is mostly seen in students engage in biological researches belonging to the life sciences or applied sciences discipline. Since biotechnology students are also engaged in extensive research work along with the stress associated with maintaining good grades, GPA, participating in extracurricular activities, completion of assignments, maintaining monthly attendance etc., signs of insomnia and anxiety are quiet prominent which affects their interpersonal and psychological functioning. Therefore, the aim of this study was to determine the incidence of insomnia and anxiety among the Biotechnology students of BUITEMS. Method: This study was conducted in BUITEMS among the biotechnology students. Athens insomnia scale with sensitivity and specificity of 93% and 85% respectively and Hamilton Anxiety Scale with sensitivity and specificity of 85.7% and 63.5% respectively were used. Besides that demographic information like age, gender etc. was also obtained from the participants. Result: The incidence of anxiety was found to be prevalent in females 66.66% than in males 63.41% and insomnia was found to be more prevalent in males 75.60% than in females 63.15%. Conclusions: Therefore, the factors that contribute to increase anxiety and insomnia should be identified and ways should be devised to reduce them.